Take a screen shot of a specif area and save as variable

I want to make a program taking a cropped screenshot (like Windows+Shift+s) and save it as variable, not as a file.

1. How do I Programmatically open this win+shift+s window?

2. How to directly get the output without saving this as a picture?

   MEDevel.com: Open-source for Healthcare and Education

   Collecting and validating open-source software for healthcare, education, enterprise, development, medical imaging, medical records, and digital pathology.

   Visit Medevel

I’m using python

>Solution :

you will need to:

1. trigger the Print screen key (any keyboard library should allow it)
2. retrieve the data from the clipboard (you will maybe forced to save it as a temporary file and then read from it)
3. then parse the data it will be it will png format.